cell line expressing cd22 Search Results


cho k1 cells  (BPS Bioscience)
91
BPS Bioscience cho k1 cells
Effect of Rubus coreanus extract (RCE) on programmed cell death protein 1 (PD-1)/PD-1 ligand-1 (PD-L1) protein interaction using competitive enzyme-linked immunosorbent assay (ELISA) or cell-based assay. ( A , B ) The effect of RCE on the binding of PD-1 and PD-L1 in vitro using a competitive ELISA assay. ( C , D ) The effect of RCE on cell viability of PD-1/NFAT Jurkat cells ( C ) and PD-L1/aAPC <t>CHO-K1</t> cells ( E ) using the Cell Counting Kit-8 (CCK) assay. Each of the cells were treated according to the indicated concentrations of RCE for 24 h. ( E ) The effect of RCE on cellular PD-1/PD-L1 blockade activity. PD-1/NFAT Jurkat effector cells and PD-L1/aAPC CHO-K1 target cells were co-cultured with RCE or αPD-L1 for 24 h at the indicated concentrations. The relative PD-1/PD-L1 blockade activity were measured by PD-1/NFAT luciferase reporter assay. ( F ) Effect of RCE on IL-2 cytokine release in co-cultured cell model. Cell culture media was analyzed to measure IL-2 level by cytokine ELISA. Data are presented as mean ± S.E. of three representative independent experiments. Asterisks indicate significant inhibition of PD-1/PD-L1 binding activity by each test inhibitor as compared with the vehicle-treated group; *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the vehicle group.
Cho K1 Cells, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+expressing+cd22/CD22+%2F+Luciferase+-+CHO+Recombinant+Cell+Line/pmc07693366-45-13-30
Average 91 stars, based on 1 article reviews
cho k1 cells - by Bioz Stars, 2026-09
91/100 stars
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cd22 cho recombinant cell line  (BPS Bioscience)
N/A
Recombinant clonal stable CHO cell line constitutively expressing full length human CD22 protein Genbank NM 001771 Surface expression of CD22 was confirmed by flow cytometry This clonal cell line was selected for medium level expression
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human cd22 knockdown cell line  (AcceGen Biotechnology)
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Human CD22 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. AcceGen offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting
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human cd22 knockout cell line  (AcceGen Biotechnology)
N/A
Human CD22 knockout cell line is edited by CRISPR/Cas9 technology.
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Image Search Results


Effect of Rubus coreanus extract (RCE) on programmed cell death protein 1 (PD-1)/PD-1 ligand-1 (PD-L1) protein interaction using competitive enzyme-linked immunosorbent assay (ELISA) or cell-based assay. ( A , B ) The effect of RCE on the binding of PD-1 and PD-L1 in vitro using a competitive ELISA assay. ( C , D ) The effect of RCE on cell viability of PD-1/NFAT Jurkat cells ( C ) and PD-L1/aAPC CHO-K1 cells ( E ) using the Cell Counting Kit-8 (CCK) assay. Each of the cells were treated according to the indicated concentrations of RCE for 24 h. ( E ) The effect of RCE on cellular PD-1/PD-L1 blockade activity. PD-1/NFAT Jurkat effector cells and PD-L1/aAPC CHO-K1 target cells were co-cultured with RCE or αPD-L1 for 24 h at the indicated concentrations. The relative PD-1/PD-L1 blockade activity were measured by PD-1/NFAT luciferase reporter assay. ( F ) Effect of RCE on IL-2 cytokine release in co-cultured cell model. Cell culture media was analyzed to measure IL-2 level by cytokine ELISA. Data are presented as mean ± S.E. of three representative independent experiments. Asterisks indicate significant inhibition of PD-1/PD-L1 binding activity by each test inhibitor as compared with the vehicle-treated group; *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the vehicle group.

Journal: Foods

Article Title: Unripe Black Raspberry ( Rubus coreanus Miquel) Extract and Its Constitute, Ellagic Acid Induces T Cell Activation and Antitumor Immunity by Blocking PD-1/PD-L1 Interaction

doi: 10.3390/foods9111590

Figure Lengend Snippet: Effect of Rubus coreanus extract (RCE) on programmed cell death protein 1 (PD-1)/PD-1 ligand-1 (PD-L1) protein interaction using competitive enzyme-linked immunosorbent assay (ELISA) or cell-based assay. ( A , B ) The effect of RCE on the binding of PD-1 and PD-L1 in vitro using a competitive ELISA assay. ( C , D ) The effect of RCE on cell viability of PD-1/NFAT Jurkat cells ( C ) and PD-L1/aAPC CHO-K1 cells ( E ) using the Cell Counting Kit-8 (CCK) assay. Each of the cells were treated according to the indicated concentrations of RCE for 24 h. ( E ) The effect of RCE on cellular PD-1/PD-L1 blockade activity. PD-1/NFAT Jurkat effector cells and PD-L1/aAPC CHO-K1 target cells were co-cultured with RCE or αPD-L1 for 24 h at the indicated concentrations. The relative PD-1/PD-L1 blockade activity were measured by PD-1/NFAT luciferase reporter assay. ( F ) Effect of RCE on IL-2 cytokine release in co-cultured cell model. Cell culture media was analyzed to measure IL-2 level by cytokine ELISA. Data are presented as mean ± S.E. of three representative independent experiments. Asterisks indicate significant inhibition of PD-1/PD-L1 binding activity by each test inhibitor as compared with the vehicle-treated group; *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the vehicle group.

Article Snippet: Jurkat cells expressing human PD-1 and NFAT reporter gene (PD-1/NFAT Jurkat cells) and CHO-K1 cells expressing human PD-L1 and a T cell receptor activator (PD-L1/aAPC CHO-K1 cells) were purchased from BPS Bioscience (San Diego, CA, USA).

Techniques: Competitive ELISA, Enzyme-linked Immunosorbent Assay, Cell Based Assay, Binding Assay, In Vitro, Cell Counting, Activity Assay, Cell Culture, Luciferase, Reporter Assay, Inhibition

Effect of ellagic acid on PD-1/PD-L1 interaction in vitro. ( A ) Effect of ellagic acid on PD-1/PD-L1 interaction was measured by competitive ELISA. Ellagic acid was treated as indicated concentration (0–120.9 μg/mL). ( B ) Ellagic acid binds to PD-1 and PD-L1. Pull-down assays were performed to detect the binding of ellagic acid with PD-1 or PD-L1. Binding by ellagic acid between human recombinant PD-1 or PD-L1 proteins were confirmed by Western blot using antibodies against PD-1 (upper panel) or PD-L1 (lower panel). Lane 1: Recombinant PD-1 or PD-L1 protein alone; lane 2: each of the proteins were precipitated with Sepharose 4B-alone; lane 3: each of the proteins were precipitated with ellagic acid–Sepharose 4B beads (EA-Sepharose 4B). ( C , D ) Effect of ellagic acid on cell viability of (c) PD-1/NFAT Jurkat cells or ( D ) PD-L1/aAPC PD-L1 CHO-K1 cells. The cell viabilities were analyzed using the Cell Counting Kit-8 (CCK) assay. Cells were treated with or ellagic acid as indicated concentrations (0–120.9 μg/mL). ( E ) Effect of ellagic acid on cellular PD-1/PD-L1 blockade activity. PD-1/NFAT Jurkat effector cells and PD-L1/aAPC CHO-K1 target cells were co-cultured with ellagic acid for 48 h at the indicated concentrations (0–7.56 μg/mL). The relative PD-1/PD-L1 blockade activity were measured by PD-1/NFAT luciferase reporter assay. ( F ) Effect of ellagic acid on IL-2 cytokine release in co-cultured cell model. Cell culture media was analyzed to measure IL-2 level by cytokine ELISA. Data are presented as mean ± S.E. of three representative independent experiments. Asterisks indicate significant inhibition of PD-1/PD-L1 binding activity by each test inhibitor as compared with the vehicle-treated group; *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the vehicle group.

Journal: Foods

Article Title: Unripe Black Raspberry ( Rubus coreanus Miquel) Extract and Its Constitute, Ellagic Acid Induces T Cell Activation and Antitumor Immunity by Blocking PD-1/PD-L1 Interaction

doi: 10.3390/foods9111590

Figure Lengend Snippet: Effect of ellagic acid on PD-1/PD-L1 interaction in vitro. ( A ) Effect of ellagic acid on PD-1/PD-L1 interaction was measured by competitive ELISA. Ellagic acid was treated as indicated concentration (0–120.9 μg/mL). ( B ) Ellagic acid binds to PD-1 and PD-L1. Pull-down assays were performed to detect the binding of ellagic acid with PD-1 or PD-L1. Binding by ellagic acid between human recombinant PD-1 or PD-L1 proteins were confirmed by Western blot using antibodies against PD-1 (upper panel) or PD-L1 (lower panel). Lane 1: Recombinant PD-1 or PD-L1 protein alone; lane 2: each of the proteins were precipitated with Sepharose 4B-alone; lane 3: each of the proteins were precipitated with ellagic acid–Sepharose 4B beads (EA-Sepharose 4B). ( C , D ) Effect of ellagic acid on cell viability of (c) PD-1/NFAT Jurkat cells or ( D ) PD-L1/aAPC PD-L1 CHO-K1 cells. The cell viabilities were analyzed using the Cell Counting Kit-8 (CCK) assay. Cells were treated with or ellagic acid as indicated concentrations (0–120.9 μg/mL). ( E ) Effect of ellagic acid on cellular PD-1/PD-L1 blockade activity. PD-1/NFAT Jurkat effector cells and PD-L1/aAPC CHO-K1 target cells were co-cultured with ellagic acid for 48 h at the indicated concentrations (0–7.56 μg/mL). The relative PD-1/PD-L1 blockade activity were measured by PD-1/NFAT luciferase reporter assay. ( F ) Effect of ellagic acid on IL-2 cytokine release in co-cultured cell model. Cell culture media was analyzed to measure IL-2 level by cytokine ELISA. Data are presented as mean ± S.E. of three representative independent experiments. Asterisks indicate significant inhibition of PD-1/PD-L1 binding activity by each test inhibitor as compared with the vehicle-treated group; *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the vehicle group.

Article Snippet: Jurkat cells expressing human PD-1 and NFAT reporter gene (PD-1/NFAT Jurkat cells) and CHO-K1 cells expressing human PD-L1 and a T cell receptor activator (PD-L1/aAPC CHO-K1 cells) were purchased from BPS Bioscience (San Diego, CA, USA).

Techniques: In Vitro, Competitive ELISA, Concentration Assay, Binding Assay, Recombinant, Western Blot, Cell Counting, Activity Assay, Cell Culture, Luciferase, Reporter Assay, Enzyme-linked Immunosorbent Assay, Inhibition